Description

BioNTech is seeking a highly motivated Scientist to join our Proteomics team. The successful candidate will work independently and as part of a team to help develop, implement and apply state of the art proteomics technologies for the detection, identification and quantification of naturally processed and presented peptide antigens across oncology and infectious disease programs.  The candidate will be part of a cross-functional team made up of immunologists, biochemists, clinicians and computational biologists whose goal is to design and contribute to novel immunotherapies.

Responsibilities

• Sample preparation for nanoLC-MS/MS analysis, including desalting, chemical modification, fractionation, and isobaric tag labeling
• Optimization and execution of immunopurification and other enrichment strategies
• Generation of biological samples via mammalian cell culture
• Method development and implementation of protein purification strategies for isolation of membrane and intracellular proteins from cells and tissues
• Routine instrument maintenance and benchmarking (Thermo and Bruker instruments)
• nanoLC-MS/MS analyses of biologically derived protein and peptide mixtures
• Analysis of nanoLC-MS/MS data and post-processing of data
• Contributions to nanoLC-MS/MS experimental design
• Review of scientific literature and collaboration with cross-functional teams
• Tracking and compiling of results in an electronic notebook
• Assisting with training of lab personnel
• Presentation of results to cross-functional teams

Requirements

Requirements

• PhD degree and experience in proteomics, mass spectrometry, biochemistry, and/or analytical chemistry
• Hands on experience with nano LC-MS/MS workflows for data acquisition including unbiased (e.g. DDA, DIA) and targeted (e.g. PRM, IS-PRM) data collection and MS-based quantitative assays, and ability to modify such methods
• Background in biochemistry, cell biology, molecular biology, biotechnology and immunology
• Experience with mammalian cell culture, including transfection and lentiviral transduction
• Strong familiarity with proteomic wet-lab protocols (immunoprecipitation, enzymatic digestion of proteins, chemical modification of peptides such as iTRAQ/TMT, desalting, fractionation, isobaric tag labeling, etc.) and ability to troubleshoot/modify such protocols.
• Familiarity with biochemical purification techniques, such as solid phase extraction, ultracentrifugation, and chromatography
• Experience with data processing workflows, such as database searching programs (e.g. Proteome Discoverer) and quantitative software (e.g. Skyline) or equivalent software
• Excellent attention to detail, organization, and record-keeping skills
• Ability to maintain inventories of equipment, supplies, and related research materials
• Ability to work independently as well as part of a collaborative, matrix environment
• Independent, flexible, self-motivated, curious, and the ability to work well with others

Optional skills

• Exposure to R or other statistical programming tools
• Exposure to software for DIA analysis (Spectronaut, DIA-NN, EncyclopeDIA, Skyline, or equivalent)